To facilitate the most advanced approaches to research, UQCCR has acquired cutting edge equipment that is user friendly and comes with local technical support. 

Hamilton Robotics

The Hamilton STAR automated liquid handling system is an open platform that can be set up to perform just about any liquid handling task you require. It uses air displacement pipetting technology and our workstation includes 8 pipette channels with the option to upgrade to a 96-channel head in future.

Our setup also currently includes:

  • two gripper options for moving microtitre plates between positions,
  • a vacuum manifold for filter plates, and
  • two heated shakers.

This system can be upgraded in future to include such options as a HEPA-filter hood, centrifuge, and evaporator; with these options the entire sample preparation process can be automated for mass spectrometry.

The advantage of using robotics for routine sample preparation goes beyond the time it frees up for you to do other tasks, it also enables much greater accuracy and precision – and opens up options such as the use of 384-well plates.

Nanosight NS500

The NanoSight NS500 Instrument provides an easy-to-use, reproducible platform for specific and general nanoparticle characterization.

With the NS500 you can analyze the presence, size distribution, concentration and fluorescence of all types of nanoparticles from 10nm to 2000nm.

Samples are introduced into the viewing chamber using the on-board fluidics capability. The on-screen pump control allows for automatic sample handling, including loading, washing and dilution. The computer-controlled motorized optical stage enables rapid relocation and refocusing of the sample and sample temperature is fully programmable through the NTA Software Suite.

The NS500 is proven with most nanoparticle classes down to 10nm (dependent upon particle type) dispersed in a wide range of solvents, for example:

  • Microvesicles and exosomes
  • Pharmaceutical nanoparticles - liposomes
  • Viruses
  • Carbon nanotubes (multi-walled)
  • Colloidal suspensions and polymer nanoparticles
  •  Nanotoxicology studies

Bio-Plex 200 System

The Bio-Plex 200 system is a suspension array system which offers protein and nucleic acid researchers with a reliable multiplex assay solution that permits the analysis of up to 100 biomolecules in a single sample. Benefits are short assay time (3-4 hours), low sample volume (50ul) and better sensitivity and dynamic range compared to ELISA.

A number of pre-mixed magnetic bead-based assays are available from Bio-Rad and provide robust and reproducible measurement of proteins involved in a broad range of research areas including:

  • Cancer
  • Immunology/Inflammation
  • Cell Signalling
  • Kidney Toxicology
  • Metabolic

Dissolved Oxygen Probe

The NeoFox Sport handheld fluorescence-based optical oxygen sensing system can be used to measure dissolved and gaseous oxygen pressure. The NeoFox Sport oxygen sensor includes a phase fluorometer, an onboard microprocessor with low-power OLED display, a user interface and a battery pack for convenient lab use.

The user interface allows you to set single- or multi-point calibration and display oxygen readings in percent of oxygen, partial pressure, moles per liter and more. Unlike polarographic and galvanic oxygen meters, the NeoFox Sport sensor is immune to environmental changes in pH, salinity and ionic strength. Plus, NeoFox Sport eliminates the need for frequent calibration. It is suitable for checking the oxygen level in your incubator or even how much is dissolved in your media.

 

LabArchives ELN

LabArchives is a web-based Electronic Lab Notebook (ELN) that is used by scientists throughout the world to store, organize, share, and publish their laboratory data. We have chosen to have it installed on a local UQ server, which provides the necessary data security for your intellectual property. It is easy and intuitive to use, yet very powerful.

The use of templates, widgets, and browser-based document editing make recording your day to day lab work quick and easy. You can try it out for free online – the only restriction is that the free version only allows 25 MB data. If you love it and want to keep using it, you can pay a small annual subscription fee per user to get up to 100 GB of space.

Gallios Flow Cytometer

The Gallios is known for its' cutting edge technology and proven performance. This instrument has fantastic detection capabilities, allowing users to visualise particles down to 0.404mm in diameter. The sensitivity and resolution of the Gallios means it can detect a variety of dyes and wavelengths. It has a customised forward scatter so users will achieve an accurate size measurement. User friendly software and fast, accurate processing allows researchers to conduct experiments efficiently.

  • 10 fluorescence detectors
  • 3 lasers
  • High speed data collection

Tecan Spark 10M Multimode Plate Reader

Absorbance with High Speed Monochromator

Spark 10M outperforms other systems with its patent pending High Speed Monochromator that delivers a spectrum in just 5 sec with a range from 200-1000 nm. Ultra-fast measurements can now be performed with unsurpassed wavelength accuracy for DNA and protein quantitation.

Fusion Optics for Fluorescence

Based on Tecan's proven Quad4™ monochromator technology, Spark 10M´s new Fusion Optics is the first optical solution to combine true monochromator flexibility with filter sensitivity for a single fluorescence measurement. Freely set a wavelength via monochromator or a filter on both the excitation and emission side. For the first time ever – enjoy sensitivity and flexibility from both the top and bottom of your sample. Polarised filters are also included to enable fluorescence polarisation measurements.

Multicolour Luminescence

Optimize your assays with Spark's multi-colour luminescence option. An excellent dynamic range of 109 by using an up to 3 OD attenuation function, and 40 user-selectable filters offer unprecedented freedom for any luminescence measurements including BRET.

StepOne Real-Time PCR System

This user-friendly device delivers precise, quantitative real-time PCR results. It collects and saves data from all filters in every run, without relying on a computer or plate set-up. Researchers can perform high resolution melt analysis and analyse data from multiple perspectives. This instrument features innovative software that is unique to the StepOne and can be used with confidence by both new and experienced researchers.

  • Perform fast PCR reactions in under 40mins
  • Fast system with plug and play assays

BioRad iQ5 Five Colour Real-Time PCR Detection System

The software available with the BioRad iQ5 real-time PCR System allows users to perform a variety of applications, including analysing gene expression of multiple genes from the same plate. The flexibility of the software also provides users with enhanced data collection and data analysis options, allowing users to have a comprehensive view of their assay results. Researchers can also easily generate publication quality graphs, tables and reports.

  • Five user-changeable filter sets which support most fluorophores
  • Powerful thermal gradient saves optimisation time
  • Performs fast PCR which reduces a researchers time to achieving results

Corbett RotorGene 6000 Real-Time PCR Instrument

The design simplicity of the RotorGene 6000 ensures maintenance is kept to a minimum, so users can focus on their research rather than waste time on the upkeep of their instrument. Air-driven heating and cooling allows for fast reactions. Greater sample-to-sample variation can also be observed. The powerful real-time software included with this instrument means it is equipped for:
 

  • High resolution melt analysis (HRM)
  • Real-time amplification
  • End-point analysis
  • Nucleic acid concentration measurement

LI-COR Odyssey Infrared Imaging System

This instrument accommodates a variety of applications, including:
 

  • Western blots
  • Cell-based imaging
  • Tissue imaging
  • Protein arrays, and more

Users attain clear and accurate data, as there is no loss of weak bands due to overexposure. Multiplex detection enables simultaneous two-colour target analysis and eliminates error. Direct detection means that no film, darkroom or messy substrates are necessary.

Xenogen IVIS Spectrum Invivo and Invitro Optical Imaging System

Used to monitor disease progression, cell trafficking and gene expression patterns in living animals, the Xenogen is the most versatile and advanced in vivo imaging system available. This instrument features advanced fluorescence and bioluminescence imaging. Spectral unmixing tools allow the user to separate signals from multiple fluorescent reporters within the same animal.

  • 3D molecular imaging
  • Transmission and reflectance imaging
  • In Vivo and In Vitro imaging

Kodak Digital X-Ray Specimen 4000 Pro System

More than 65 000 levels of grayscale resolution allows for accurate x-ray density measurement. The fully automated lens and filter systems available in this instrument together with its high resolution make it the best digital resolution available in cabinet x-ray systems.

Zeiss LSM 710 Confocal with Live Cell Imaging Module

With excellent sensitivity, this machine is used for cell imaging, single molecule analysis, and imaging minute structures (such as DNA). Offering capabilities beyond conventional imaging, the Confocal allows for Fluorescence Correlation Spectroscopy (FCS) and Image Correlation Spectroscopy (RICS). Researchers can use up to ten dyes simultaneously enabling them to look deeper into cells and tissues (e.g. brain tissue).

  • Creates detailed, high-contrast images
  • Capture light released by a single plane of a sample
  • More stable conditions for living cells mean live cell imaging results are more accurate

 

Zeiss Axio Imager M1

Known for its outstanding optics, the Zeiss Axio Imager has high resolution and excellent contrast. From single image acquisition to image analysis, researchers use this instrument for a range of imaging functions.

  • Fantastic for fluorescence imaging
  • Great for detecting parasites, bacteria or virus aggregates

 

IncuCyte

The Essen IncuCyte is an incubator microscope system for live cell imaging and cellular assays. The system allows time lapse live cell imaging of cells in a variety of vessels, including dishes, tissue culture plates, and multi-well chambers. Images can be reviewed and analyzed from a networked remote computer without opening the incubator or disturbing the cells.

The system is especially suited for monitoring cell lines (cell growth, transgene expression, cell migration), and for high content assay development. The analysis software suite includes validated assays for cell migration, scratch wound assay, apoptosis, cell proliferation, angiogenesis, reporter gene expression, and more.

The system includes:

  • 10x Nikon objectives
  • Enhanced phase contrast and GFP fluorescence
  • 30 TB data server
  • Incucyte image viewing and analysis software. Includes many validated assays.

Accepts micro-plates (96, 48, 24, 12, and 6 well), culture flasks (T25, T75, T175), dishes (35mm and 90mm), and slide chambers.

Imaging Specifications:

  • Image Resolution: 1.49 microns
  • Image Format: 1280 x 1024 pixels
  • Field of View: 1.90 x 1.52 mm
  • Magnification: 10x

FLR Optical Configuration:

  • Emission Filter Wavelength: 515 nm
  • Emission Filter Name: 515BP30
  • Excitation Wavelength: 470 nm
  • Excitation Filter Name: 470BP40
  • Dichroic Filter Name: 495LP

MultiNA

The Shimadzu MCE-202 MultiNA  is a completely automated high-speed microchip-based electrophoresis system for analysis of nucleic acids.  Both quantitation and fragment size determinations of up to 108 samples are rapidly performed over a wide dynamic range in a single analysis.

NanoMate

The TriVersa NanoMate utilizes chip-based electrospray technology to enhance the ability of LC/MS to analyse complex samples.  Associated with CCD’s LC/MS facility, the NanoMate has three different functionalities:

  1. Chip-based infusion using an integrated nanoelectrospray microchip containing an array of 400 emitters allowing flow rates between 20 and 300 nL/min.  This allows a long, stable spray, and eliminates sample-to-sample carryover.
  2. LC/MS fraction collection/infusion couples a conventional HPLC system to the NanoMate, with the HPLC effluent split between a conventional LC/MS interface, and collection into a multi-well plate, allowing fractions of interest to be selected for more in-depth analysis post-LC/MS.  This is suited to metabolite identification, biomarker analysis, or protein characterisation.
  3. Liquid Extraction Surface Analysis (LESA) allows direct nanoESI mass spectrometric analysis from any surface with extractable analytes, such as thin tissue sections, dried blood spots on paper, or MALDI plates.

QTRAP® 5500 System

The AB SCIEX QTRAP® 5500 LC/MS/MS System is a triple quadrupole/linear ion trap (LIT) Mass Spectrometer designed for biomarker and metabolite identification, quantitation and validation, especially in proteomics and lipidomics. 

The QTRAP® 5500 System houses the most sensitive ion trap in the world, along with offering ultra-fast scan speeds and full MS capabilities.

TripleTOF® 5600 System

The AB Sciex TripleTOF® 5600 System is an LCMSMS system that uniquely integrates comprehensive, qualitative exploration, rapid profiling, and high-resolution quantitation workflows on a single platform. It combines high-sensitivity detection, high resolution with the fast acquisition speeds, and stable mass accuracy over days of acquisition. The 5600 System provides a new paradigm in proteomics research.

TOF/TOF™ 5800 System

The AB SCIEX TOF/TOF™ 5800 System provides the fastest and most confident path to identification and relative quantitation of proteins. The system's unmatched speed and sensitivity make it the ideal platform for biomarker discovery, MALDI mass spectrometry imaging and protein identification.

  • More than 10-fold faster data acquisition with new 1 KHz laser and DynamicExit™ Algorithm
  • Unique OptiBeam™ On-axis Laser irradiation increases sensitivity in both MS and MS/MS modes by 10-fold over systems without an on-axis laser
  • Maximum up-time and throughput by continuously heating of the source mirror and user-programmable source mirror cleaning
  • Improved resolution with new reflectron mirror design and 1000 MHz digitizer
  • High-resolution precursor ion selection with novel timed-ion selector.

LCMS-8050 Triple Quadrupole Liquid Chromatograph Mass Spectrometer (LC-MS/MS)

Product by Shimadzu. Enhancements in chromatography and sub-2 micron columns have caused narrower peak widths, increasing resolution. In addition to high-resolution columns, analysts are demanding shorter chromatographic run times while adding more analytes to a single method. With these two factors at play, it is clear that the LCMS-8050 ultra fast mass spectrometer is needed in order to accurately and reliably detect each compound in a sample.